Alkaline Phosphatase Activity as a Predictive Marker of Osteogenesis in Human Bone Marrow-derived Mesenchymal Stem Cells but Not Umbilical Cord Blood-derived Mesenchymal Stem Cells
نویسندگان
چکیده
INTRODUCTION Mesenchymal stem cells (MSCs) are defined as cells that can differentiate into multiple mesenchymal lineage cells. MSCs were obtained from various sources; bone marrow, umbilical cord blood, adipose tissue and synovium etc. It has been reported that MSCs isolated from the bone marrow showed variation in size, morphology and level of alkaline phosphatase activity among clones isolated from a donor. Expression of alkaline phosphatase (ALP) is widely accepted as an early marker of osteogenesis in bone-forming system. In addition, the enzyme express positively in mouse embryonic stem cells. However, there is no unequivocal evidence indicating the role of ALP in human MSCs. Therefore, the aim of this study was to characterize the correlation between alkaline phosphatase activity and differentiation ability of human bone marrow-derived mesenchymal stem cell (hBMSC) and umbilical cord blood (UCB)-derived MSCs in vitro.
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